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ANC Workshop Chris Williams/Sohan Seth Chairing: Thomas Suslak

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  • ANC Workshop Talk
When Jan 27, 2015
from 11:00 AM to 12:00 PM
Where Room 4.31/4.33
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Chris Williams

Title:  Localisation microscopy with quantum dots using non-negative matrix

Abstract:  We propose non-negative matrix factorisation with iterative
restarts (iNMF) to model a noisy dataset of highly overlapping fluorophores
with intermittent intensities. We can recover high-resolution images of
individual sources from the optimised model, despite their high mutual
overlap in the original data. Each source can have an arbitrary, unknown
shape of the PSF and blinking behaviour. This allows us to use quantum dots
as bright and stable fluorophores for localisation microscopy. We compare
the iNMF results to CSSTORM, 3B and bSOFI. iNMF shows superior
performance in the challenging task of the super-resolution imaging using
quantum dots. We can also retrieve axial localisation of the sources from
the shape of the recovered PSF. Joint work with Ondrej
Mandula, Ivana Sumanovac Sestak and Rainer Heintzmann. 

Seth Sohan

Title: PROTEUS: Multiplexed Optical Molecular Sensing and Imaging


Abstract: Critically ill patients at the ICU require real time diagnosis of physiological

and pathological changes in the lungs. A prompt diagnosis facilitates

administration of the right drug in the right amount to avoid infection that

can be otherwise life threatening. PROTEUS is an EPSRC funded research

consortium of three universities, University of Edinburgh, University of Bath,

and Heriot Watt University, aiming to build a device for this purpose.  This

device will comprise optical fibres, with molecular sensors attached at the

end, that can be send down patient's lung to measure physiological

parameters. This device will also be capable of taking images inside the lung,

and, if needed, deliver drugs. I will give a brief overview of this project, and

discuss some ongoing work on predicting pH level from surface-enhanced Raman spectroscopy signals.