Adrianna Teriakidis PhD

Adrianna Teriakidis


Publications:
2014
  Analysis of local and global topographic order in mouse retinocollicular maps
Willshaw, DJ, Sterratt, DC & Teriakidis, A 2014, 'Analysis of local and global topographic order in mouse retinocollicular maps' Journal of Neuroscience, vol 34, no. 5, pp. 1791-805. DOI: 10.1523/JNEUROSCI.5602-12.2014
We introduce the Lattice Method for the quantitative assessment of the topographic order within the pattern of connections between two structures. We apply this method to published visuocollicular mapping data obtained by Fourier-based intrinsic imaging of mouse colliculus. We find that, in maps from wild types and ?2 knock-outs, at least 150 points on the colliculus are represented in the visual field in the correct relative order. In maps from animals with knock-out of the three ephrinA ligands (TKO), thought to specify the rostrocaudal axis of the map, the projection on the colliculus of each small circular area of visual field is elongated approximately rostrocaudally. Of these projections, 9% are made up of two distinct regions lying along the direction of ingrowth of retinal fibers. These are similar to the ectopic projections found in other ephrinA knock-out data. Coexisting with the ectopic projections, each TKO map contains a submap where neighbor-neighbor relations are preserved, which is ordered along both rostrocaudal and mediolateral axes, in the orientation found in wild-type maps. The submaps vary in size with order well above chance level, which can approach the order in wild-type maps. Knock-out of both ?2 and two of the three ephrinAs yields maps with some order. The ordered TKO maps cannot be produced by correlated neural activity acting alone, as this mechanism is unable to specify map orientation. These results invite reassessment of the role of molecular signaling, particularly that of ephrinAs, in the formation of ordered nerve connections.
General Information
Organisations: School of Informatics.
Authors: Willshaw, David J, Sterratt, David C & Teriakidis, Adrianna.
Keywords: (Animals, Brain Mapping, Ephrin-B2, Fourier Analysis, Mice, Mice, Knockout, Neuroimaging, Receptors, Eph Family, Retina, Superior Colliculi, Visual Fields, Visual Pathways. )
Number of pages: 15
Pages: 1791-805
Publication Date: 29 Jan 2014
Publication Information
Category: Article
Journal: Journal of Neuroscience
Volume: 34
Issue number: 5
ISSN: 0270-6474
Original Language: English
DOIs: 10.1523/JNEUROSCI.5602-12.2014
2012
  Prevalence and elimination of sibling neurite convergence in motor units supplying neonatal and adult mouse skeletal muscle
Teriakidis, A, Willshaw, DJ & Ribchester, RR 2012, 'Prevalence and elimination of sibling neurite convergence in motor units supplying neonatal and adult mouse skeletal muscle' Journal of Comparative Neurology, vol 520, no. 14, pp. 3203-3216. DOI: 10.1002/cne.23091
During development, neurons form supernumerary synapses, most of which are selectively pruned leading to stereotyped patterns of innervation. During the development of skeletal muscle innervation, or its regeneration after nerve injury, each muscle fiber is transiently innervated by multiple motor axon branches but eventually by a single branch. The selective elimination of all but one branch is the result of competition between the converging arbors. It is thought that motor neurons initially innervate muscle fibers randomly, but that axon branches from the same neuron (sibling branches) do not converge to innervate the same muscle fiber. However, random innervation would result in many neonatal endplates that are co-innervated by sibling branches. To investigate whether this occurs we examined neonatal levator auris longus (LAL) and 4th deep lumbrical (4DL) muscles, as well as adult reinnervated deep lumbrical muscles (1?4) in transgenic mice expressing yellow fluorescent protein (YFP) as a reporter. We provide direct evidence of convergence of sibling neurites within single fluorescent motor units, both during development and during regeneration after nerve crush. The incidence of sibling neurite convergence was 40% lower in regeneration and at least 75% lower during development than expected by chance. Therefore, there must be a mechanism that decreases the probability of its occurrence. As sibling neurite convergence is not seen in normal adults, or at later timepoints in regeneration, synapse elimination must also remove convergent synaptic inputs derived from the same motor neuron. Mechanistic theories of synaptic competition should now accommodate this form of isoaxonal plasticity.
General Information
Organisations: Centre for Integrative Physiology.
Authors: Teriakidis, Adrianna, Willshaw, David J. & Ribchester, Richard R..
Keywords: (neuromuscular junction , development, reinnervation, , , . )
Number of pages: 14
Pages: 3203-3216
Publication Date: Oct 2012
Publication Information
Category: Article
Journal: Journal of Comparative Neurology
Volume: 520
Issue number: 14
ISSN: 1096-9861
Original Language: English
DOIs: 10.1002/cne.23091
2010
  Algorithms for automatic neuron tracing in noisy 3D image stacks
Knowles-Barley, S, Phillipson, G, Teriakidis, A, Sterratt, D, Larkworthy, T, O'Leary, T, Down, MP, Hennig, M, Withers, JPJ & Hull, M 2010, 'Algorithms for automatic neuron tracing in noisy 3D image stacks' Neuroscience Day 2010, Edinubrgh, United Kingdom, 29/03/10 - 29/03/10, .
General Information
Organisations: Institute for Adaptive and Neural Computation .
Authors: Knowles-Barley, Seymour, Phillipson, Graeme, Teriakidis, Adrianna, Sterratt, David, Larkworthy, Thomas, O'Leary, Timothy, Down, Matthew P., Hennig, Matthias, Withers, James P. J. & Hull, Michael.
Publication Date: 2010
Publication Information
Category: Poster
Original Language: English
  GPU Computing for 3D Data Annotation
Knowles-Barley, S, Teriakidis, A, Phillipson, G, Sterratt, D & Willshaw, D 2010, 'GPU Computing for 3D Data Annotation'.
A quick, flexible, accurate and automatic volumetric image processing software package will enable biomedical researchers to analyse orders of magnitude more data. In particular this could have a huge impact on the advancement of the field of neuroscience and our understanding of the brain. For certain operations Graphics Processing Unit (GPU) processing is many times faster than performing the same operations on a standard computer Central Processing Unit (CPU).
General Information
Organisations: Institute for Adaptive and Neural Computation .
Authors: Knowles-Barley, Seymour, Teriakidis, Adrianna, Phillipson, Graeme, Sterratt, David & Willshaw, David.
Publication Date: 2010
Publication Information
Category: Poster
Original Language: English
2007
  Competitive, intrinsic and stochastic determinants of neuromuscular synapse elimination in transgenic YFP expressing mice
Teriakidis, A, Jenkins, N, Willshaw, D & Ribchester, R 2007, 'Competitive, intrinsic and stochastic determinants of neuromuscular synapse elimination in transgenic YFP expressing mice' 19th Annual Meeting of the British Neuroscience Association (BNA) , Harrogate, United Kingdom, 1/04/07 - 4/04/07, .
Competition at motor endplates strongly influences elimination of polyneuronal innervation in neonatal muscles but it is still not known whether intrinsic prope rties of motor neurones also determine ultimate motor unit size. We are using transgenic mice expressing fluorescent protein in motor neurones to measure and model the changes in motor unit size as neuromuscular synapses form or become eliminated. First we designed experiments to test whether motor unit size in adult lumbrical muscles becomes reduced even when all other competing motor units are removed, by partial denervation at birth (neonates anaesthetised by chilling). We found that motor unit size was not reduced when these mice reached adulthood, suggesting that competition is sufficient to account for synapse elimination during development. Next we examined whether the initial motor innervation pattern is random. Analysis of YFP -expressing motor units in lumbrical muscles of thy1.2 -YFPH mice indicated that all connections made by a motor neurone are restricted to a single lumbrical muscle with no divergence between muscles. Stochastic modeling suggested that a key indicator of randomness is initial converge of branches of the same axon on single motor endplates. To test this we examined adult muscles in the earliest stages of reinnervation (12-14 days) after nerve crush under halothane/N20 anaesthesia. We found several compelling instances of within-unit convergence on motor endplates. Thus, stochastic properties play an important role in establishing the divergent and convergent innervation pattern within muscles but competitive interactions at polyinnervated junctions are more important than intrinsic properties of neurones during synapse elimination.
General Information
Organisations: Centre for Integrative Physiology.
Authors: Teriakidis, Adrianna, Jenkins, Nia, Willshaw, David & Ribchester, Richard.
Publication Date: 2007
Publication Information
Category: Poster
Original Language: English
2006
  Competition vs Intrinsic Withdrawal in the development of the NMJ
Teriakidis, A & Ribchester, R 2006, 'Competition vs Intrinsic Withdrawal in the development of the NMJ'.
At birth many muscle fibres are innervated by more than one motor neuron. During development all but one of these connections are lost, leaving each muscle fibre innervated by a single axon.
Synapse elimination is thought to result from competition between axon terminals innervating the same end-plate. There is some debate as to whether there is also an 'intrinsic withdrawal' component in synapse elimination. Thompson & Jansen (1977) and Fladby & Jansen (1987) found, in rat and mouse soleus muscles respectively, that motor unit size decreases during development even in the absence of competition resulting in the complete denervation of some muscle fibres. Betz et al. (1980) and Fisher et al. (1989) found no such decrease in rat 4th deep lumbrical (4DL) and rat soleus muscles respectively.
Until now the methods available for estimating motor unit size have been indirect and inherently inaccurate. Now it is possible to directly visualise motor axons and end-plates and thus it is possible to obtain a more direct measurement of average motor unit size during development.
General Information
Organisations: Centre for Integrative Physiology.
Authors: Teriakidis, Adrianna & Ribchester, Richard.
Publication Date: 2006
Publication Information
Category: Poster
Original Language: English

Projects:
What are the contributions of competition and intrinsic withdrawal in synapse elimination during development (PhD)